The quantitative microleakage of class V cementum (dentin) cavities re
stored with six dentinal bonding restorative systems was determined in
vitro. Ninety extracted human permanent first and second mandibular a
nd maxillary premolars were used in this study. Class V preparations w
ere made in cementum (dentin) at the root facial surfaces. The prepara
tions were restored with 1) a dentin bonding system containing 2% HEMA
and BisGMA and a light-cured microfilled composite; 2) the same mater
ials only substituting META/MMA base and TBB catalyst monomers for the
BisGMA sealer; 3) a dentin bonding system containing 35% HEMA with ME
TA/MMA base and TBB catalyst, and a light-cured microfilled composite;
4) the same materials with an experimental light-cured hybrid composi
te; 5) the same dentin bonding system only substituting the 35% glycer
yl methacrylate for the 35% HEMA and using the microfilled composite;
and 6) the previously described system with a substitution of 0.5 mol
EDTA for the 10% citric acid -3% FeCl3. Fifteen teeth were restored wi
th each procedure. The restorations were finished with 12-bladed carbi
de burs 15 min after placement, the teeth were stored in saline at 37-
degrees-C for 24 h, finished with Sof-Lex discs and then thermocycled
in 2% methylene blue solution 500 times between 50-degrees-C and 8-deg
rees-C with a dwell time of 15 s. Quantitative microleakage was determ
ined by a spectrophotometric dye-recovery method and expressed in mug/
dye/restoration. The data were analyzed by ANOVA, Student-Newman-Keuls
and Kruskal-Wallis tests. The quantitative microleakage of the teeth
restored with the adhesive systems containing 35% glyceryl methacrylat
e was significantly reduced. The bonding mechanism of glyceryl methacr
ylate is not known.