DETECTION OF EPHEDRINE AND PHENYLPROPANOLAMINE IN URINE USING A POLARIZATION FLUOROIMMUNOASSAY

Authors
EREMIN SA SMIRNOV AV GALLACHER G SMITH DS COLBERT DL
Citation
Sa. Eremin et al., DETECTION OF EPHEDRINE AND PHENYLPROPANOLAMINE IN URINE USING A POLARIZATION FLUOROIMMUNOASSAY, Analyst, 118(10), 1993, pp. 1325-1328
Citations number
10
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
118
Issue
10
Year of publication
1993
Pages
1325 - 1328
Database
ISI
SICI code
0003-2654(1993)118:10<1325:DOEAPI>2.0.ZU;2-4
Abstract
A polarization fluoroimmunoassay, with a high specificity for ephedrin e and phenylpropanolamine, has been adapted for screening urine sample s on the Abbott TDx analyser. The antiserum was produced from an ephed rine derivative conjugated to keyhole limpet haemocyanin, and, as a tr acer, a fluorescein-labelled derivative of phenylpropanolamine was use d. The antiserum showed very low cross-reactivity for amphetamine and other structurally related amines.