RIBONUCLEOTIDE REDUCTASE - A POSSIBLE TARGET FOR OROTIC-ACID INDUCED MITOINHIBITION IN NORMAL HEPATOCYTES IN PRIMARY CULTURE

Authors
MANJESHWAR S PICHIRICONI G CONI P RAO PM RAJALAKSHMI S SARMA DSR
Citation
S. Manjeshwar et al., RIBONUCLEOTIDE REDUCTASE - A POSSIBLE TARGET FOR OROTIC-ACID INDUCED MITOINHIBITION IN NORMAL HEPATOCYTES IN PRIMARY CULTURE, Cancer letters, 73(2-3), 1993, pp. 149-154
Citations number
24
Categorie Soggetti
Oncology
Journal title
Cancer letters → ACNP
ISSN journal
03043835
Volume
73
Issue
2-3
Year of publication
1993
Pages
149 - 154
Database
ISI
SICI code
0304-3835(1993)73:2-3<149:RR-APT>2.0.ZU;2-3
Abstract
The present study was designed to determine the mechanism by which oro tic acid, a rat liver tumor promoter, inhibits DNA synthesis in normal hepatocytes in primary culture. Our results indicate that orotic acid inhibited the epidermal growth factor induced expression (mRNA) of bo th MI and M2 subunits of ribonucleotide reductase while the expression of c-fos, c-myc, c-Ha-ras and beta-actin was not inhibited to any sig nificant extent. These studies suggest that ribonucleotide reductase m ay be one target for orotic acid-induced mitoinhibition.