EFFECT OF PROLACTIN ISOLATED FROM HUMAN AMNIOTIC-FLUID ON CULTURED BOVINE CELLS OF THE REPRODUCTIVE-TRACT AND SPLENOCYTES

Prolactin (PRL) was isolated from human amniotic fluid and than fracti onated on Concanavalin A-Sepharose (ConA-S). Two fractions were tested for the H-3-thymidine incorporation in the DNA of bovine granulosa, o viductal and stromal cells as well as splenocytes. All cell types reac ted to PRL I or PRL II in a dose dependent manner. In general the effe ct of PRL I was greater than PRL II, and low concentrations of both en hanced the cell proliferation more than higher doses. The incorporatin g effect of H-3-thymidine under the influence of PRL I was higher in s tromal cells and splenocytes than in granulosa and oviductal cells. PR L II did not have such a stimulating effect, even the presence of 14 n g/ml resulted in a decrease of H-3-thymidine incorporation in granulos a cells. The reason of this different intensity of tested PRL variants may depend on the molecular heterogeneity. The results indicate that PRL forms possess a cel type-specific effect on the proliferation of c ells of the bovine female reproductive tract and splenocytes.