DIRECT DETERMINATION OF OPIUM ALKALOID-BOVINE SERUM-ALBUMIN CONJUGATEBY MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY

Authors
SHOYAMA Y FUKADA T TANAKA T KUSAI A NOJIMA K
Citation
Y. Shoyama et al., DIRECT DETERMINATION OF OPIUM ALKALOID-BOVINE SERUM-ALBUMIN CONJUGATEBY MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY, Biological & pharmaceutical bulletin, 16(10), 1993, pp. 1051-1053
Citations number
9
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Biological & pharmaceutical bulletin → ACNP
ISSN journal
09186158
Volume
16
Issue
10
Year of publication
1993
Pages
1051 - 1053
Database
ISI
SICI code
0918-6158(1993)16:10<1051:DDOOAS>2.0.ZU;2-M
Abstract
Opium alkaloids (thebaine, codeine and morphine) have been conjugated with bovine serum albumin (BSA) to give individual antigen conjugates which are analyzed by matrix-assisted laser desorption/ionization (MAL DI) mass spectrometry. It became clear that 9 molecules of thebaine we re contained in a thebaine-BSA conjugate. Codeine and morphine content s in individual conjugates were determined to be 12 and 6 molecules, r espectively.