P. Manzerra et al., TEMPORAL AND SPATIAL-DISTRIBUTION OF HEAT-SHOCK MESSENGER-RNA AND PROTEIN (HSP70) IN THE RABBIT CEREBELLUM IN RESPONSE TO HYPERTHERMIA, Journal of neuroscience research, 36(4), 1993, pp. 480-490
We have previously investigated the expression of hsp70 genes in the h
yperthermic rabbit brain at the mRNA level by Northern blot and in sit
u hybridization procedures. Our studies have now been extended to the
protein level utilizing Western blot and immunocytochemistry. Using an
antibody which is specific to inducible hsp70, a prominent induction
of hsp70 protein in glial cells of hyperthermic animals was noted. In
particular, Bergmann glial cells in the cerebellum are strongly immuno
reactive while adjacent Purkinje neurons are immunonegative. Extension
of our in situ hybridization studies to a time course analysis reveal
ed that the initial glial induction events were followed by a delayed
accumulation of inducible hsp70 mRNA in Purkinje neurons at 10 hr post
-heat shock. In control animals, high levels of constitutively express
ed hsc70 mRNA and protein were observed in Purkinje neurons. Similar h
sc70 and hsp70 mRNA observations were also made in neurons of the deep
cerebellar nuclei and in motor neurons of the spinal cord. Our result
s suggest that these neuronal cell types accumulate hsp70 mRNA in resp
onse to hyperthermic treatment; however, the response is delayed when
compared to the rapid response seen in glial cells. The high constitut
ive levels of hsc70 in certain neuronal cell types may play a role in
the initial dampening of the hsp70 induction response in these cells.
(C) 1993 Wiley-Liss, Inc.