F. Okano et al., THE DETECTION OF A BREAST-CANCER ANTIGEN ON MCF-7 CELLS REACTIVE WITHTHE TCR(ALPHA) OF A SPECIFIC KILLER T-CELL LINE, Biotherapy, 6(3), 1993, pp. 195-203
We established several immortalized human T cell lines by cotransfecti
ng primary lymphocytes derived from breast cancer patients with human
c-myc and human c-Ha-ras oncogenes. A CD8 positive (CD8+) killer T cel
l line, FT-8, exhibited in vitro specific cytotoxicity to a human brea
st cancer cell line, MCF-7. The FT-8 cells suppressed the growth of MC
F-7 cells transplanted to athymic mice. The cytotoxic reaction was cau
sed via T cell antigen receptor (TCR) on MCF-7 cells, because monoclon
al antibodies against the TCR inhibited the cytotoxicity of FT-8 cells
. The TCR(alpha) cDNA of FT-8 was cloned by using a PCR amplification
technique and expressed by a cell-free in vitro translation system. Th
e TCR(alpha) protein recognized a target antigen of 32 KDa on MCF-7 ce
lls.