THE RAT GASTRIC PHOSPHOLIPIDS - INCREASED IN ULCERATED MUCOSA AND DECREASED AFTER HEALING

The composition and content of the gastric phospholipids were followed during development and healing of indomethacin-induced chronic, antra l ulcers in rats. The individual phospholipids were identified by thin -layer chromatography and quantitatively estimated by spectrophotometr ic analysis of phosphate. No changes were found in phospholipid compos ition and content after a 24-hour fast or during the first 24 h after indomethacin was given. The total phospholipid content and the content of lysophosphatidylcholine and phosphatidylcholine were increased on the 5th day, when chronic ulcers were established. After 4 weeks, when the ulcers were healed, the total phospholipid content, and content o f phosphatidylcholine, phosphatidylethanolamine and phosphatidylinosit ol, respectively, were decreased, while the content of lysophosphatidy lcholine was increased. In conclusion, the composition and content of gastric phospholipids were altered in rats with ulcers as well as in r ats with healed ulcers.