IN-VIVO ADMINISTRATION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-SPECIFIC PEPTIDES FROM INFLUENZA-VIRUS INDUCES SPECIFIC CYTOTOXIC T-CELLHYPORESPONSIVENESS
A. Mullbacher et Rt. Hla, IN-VIVO ADMINISTRATION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-SPECIFIC PEPTIDES FROM INFLUENZA-VIRUS INDUCES SPECIFIC CYTOTOXIC T-CELLHYPORESPONSIVENESS, European Journal of Immunology, 23(10), 1993, pp. 2526-2531
We have been investigating the immunogenicity of two class I major his
tocompatibility complex-specific peptides with a sequence derived from
influenza virus nucleoprotein specific for K(d) and one for D(b). Pep
tide-modified splenocytes are unable to immunize for a primary cytotox
ic T (Tc) cell response in vivo, or secondary response in vitro. Pepti
de-modified stimulator cells can boost virus-primed splenocytes for a
strong secondary response in vitro. Animals primed with syngeneic pept
ide-modified splenocytes upon challenge with virus in vivo do not gene
rate strong secondary Tc cell responses on day 3 after challenge in co
ntrast to virus primed animals. Day 6 responses of virus-challenged, p
eptide-primed animals are reduced as compared to unprimed mice. This h
yporesponsiveness is independent of CD8+ T cells in the priming popula
tion and can be elicited with tumor cell lines. The data are discussed
in the framework of the two-signal model of immune induction.