PARTIAL SEQUENCING OF 2 FORMS OF CONCANAVALIN A-UNBOUND COLLAGENASE INHIBITOR FROM BOVINE GINGIVA

Authors
MIZUNO T IGARASHI A NOHARA H
Citation
T. Mizuno et al., PARTIAL SEQUENCING OF 2 FORMS OF CONCANAVALIN A-UNBOUND COLLAGENASE INHIBITOR FROM BOVINE GINGIVA, Archives of oral biology, 38(10), 1993, pp. 917-918
Citations number
10
Categorie Soggetti
Dentistry,Oral Surgery & Medicine
Journal title
Archives of oral biology → ACNP
ISSN journal
00039969
Volume
38
Issue
10
Year of publication
1993
Pages
917 - 918
Database
ISI
SICI code
0003-9969(1993)38:10<917:PSO2FO>2.0.ZU;2-9
Abstract
Three forms of collagenase inhibitor, one ConA-bound and two ConA-unbo und, were extensively purified from bovine gingiva by sequential colum n chromatography. Analysis by sodium dodecyl sulphate-polyacrylamide g el electrophoresis revealed that inhibitory activity resides in protei ns with M(r) of 26000-28000 and 22000 for ConA-bound and two ConA-unbo und inhibitors, respectively. Of these, two ConA-unbound inhibitors we re partially sequenced in the first 12 amino acids and found to have a n identical sequence. The NH2-terminal sequence had 100% identity with TIMP-2 or MI.