T. Mizuno et al., PARTIAL SEQUENCING OF 2 FORMS OF CONCANAVALIN A-UNBOUND COLLAGENASE INHIBITOR FROM BOVINE GINGIVA, Archives of oral biology, 38(10), 1993, pp. 917-918
Three forms of collagenase inhibitor, one ConA-bound and two ConA-unbo
und, were extensively purified from bovine gingiva by sequential colum
n chromatography. Analysis by sodium dodecyl sulphate-polyacrylamide g
el electrophoresis revealed that inhibitory activity resides in protei
ns with M(r) of 26000-28000 and 22000 for ConA-bound and two ConA-unbo
und inhibitors, respectively. Of these, two ConA-unbound inhibitors we
re partially sequenced in the first 12 amino acids and found to have a
n identical sequence. The NH2-terminal sequence had 100% identity with
TIMP-2 or MI.