L. Fulop et al., RAPID IDENTIFICATION OF FLAVIVIRUSES BASED ON CONSERVED NS5-GENE SEQUENCES, Journal of virological methods, 44(2-3), 1993, pp. 179-188
Two conserved regions in the sequence of the NS5 gene of Flaviviruses
were identified. Primers were designed from the consensus sequence of
these regions and were used in a reverse transcription/polymerase chai
n reaction (RT/PCR) to amplify a region of the central european tick-b
orne encephalitis virus Kumlinge NS5 gene. The authenticity of the amp
lified fragment was confirmed by nucleotide sequencing. A band of the
expected size was also obtained when this RT/PCR was applied to 13 oth
er flaviviral RNAs. This method may be useful for characterisation of
the NS5 genes of flaviviruses and as a potential pan-flavivirus diagno
stic tool.