BLUETONGUE VIRUS - PRODUCTION AND STUDY OF VIRAL-ANTIGEN FOR SEROLOGICAL DIAGNOSIS

Authors
ARITA GMM GATTI MSV DEAK JG DECASTRO AFP
Citation
Gmm. Arita et al., BLUETONGUE VIRUS - PRODUCTION AND STUDY OF VIRAL-ANTIGEN FOR SEROLOGICAL DIAGNOSIS, Journal of virological methods, 44(2-3), 1993, pp. 281-286
Citations number
23
Categorie Soggetti
Virology,"Biochemical Research Methods
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
44
Issue
2-3
Year of publication
1993
Pages
281 - 286
Database
ISI
SICI code
0166-0934(1993)44:2-3<281:BV-PAS>2.0.ZU;2-N
Abstract
A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated b y sequential ultrafiltration with membranes with cut-off values 10(3) and 25 x 10(3) NMWP, showed complete identity to standard antigens whe n compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, r evealing that the main protein component responsible for the AGID reac tion has a molecular weight of about 60 kDa corresponding probably to the NS1 protein.