DETECTION OF A SHARED IDIOTOPE ON 2 ENCEPHALOMYOCARDITIS VIRUS NEUTRALIZING MONOCLONAL-ANTIBODIES BY NEUTRALIZATION INHIBITION ENZYME-IMMUNOASSAY

Authors
KRAAIJEVELD CA OOSTERLAKEN TAM VLASPOLDER F VANDIJK PW HARMSEN M FERNANDEZ IM BENAISSATROUW BJ SNIPPE H
Citation
Ca. Kraaijeveld et al., DETECTION OF A SHARED IDIOTOPE ON 2 ENCEPHALOMYOCARDITIS VIRUS NEUTRALIZING MONOCLONAL-ANTIBODIES BY NEUTRALIZATION INHIBITION ENZYME-IMMUNOASSAY, Journal of virological methods, 44(2-3), 1993, pp. 319-328
Citations number
22
Categorie Soggetti
Virology,"Biochemical Research Methods
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
44
Issue
2-3
Year of publication
1993
Pages
319 - 328
Database
ISI
SICI code
0166-0934(1993)44:2-3<319:DOASIO>2.0.ZU;2-1
Abstract
Idiotypic cross-reactivity between encephalomyocarditis virus (EMCV) n eutralizing monoclonal antibodies UM 21.1 (IgG2b) and UM 21.3 (IgG2a) was detected by neutralization inhibition enzyme immunoassay using pol yclonal and monoclonal anti-idiotypic antibodies. One strongly cross-r eactive anti-idiotypic monoclonal antibody, designated 21.1A5 (IgG2b), might recognize a recurrent idiotope on EMCV neutralizing antibodies but it did not induce EMCV neutralizing anti-anti-idiotypic antibodies in homologous BALB/c mice.