AN IN-SITU HYBRIDIZATION TECHNIQUE FOR THE STUDY OF B19 HUMAN PARVOVIRUS REPLICATION IN BONE-MARROW CELL-CULTURES

Authors
VASSIAS I PEROL S COULOMBEL L THEBAULT MC LAGRANGE PH MORINET F
Citation
I. Vassias et al., AN IN-SITU HYBRIDIZATION TECHNIQUE FOR THE STUDY OF B19 HUMAN PARVOVIRUS REPLICATION IN BONE-MARROW CELL-CULTURES, Journal of virological methods, 44(2-3), 1993, pp. 329-338
Citations number
14
Categorie Soggetti
Virology,"Biochemical Research Methods
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
44
Issue
2-3
Year of publication
1993
Pages
329 - 338
Database
ISI
SICI code
0166-0934(1993)44:2-3<329:AIHTFT>2.0.ZU;2-Y
Abstract
An in situ hybridization technique using digoxigenin labelling was dev eloped to study B19 infection. By using appropriate DNA probes, transc ription of structural and non-structural genes was detected in bone ma rrow cell cultures. Such a simple system is useful to the study of B19 -cell interactions in non-permissive cell lines.