Sl. Li et al., EXPRESSION OF EPSTEIN-BARR-VIRUS-RELATED NUCLEAR ANTIGENS AND B-CELL MARKERS IN LYMPHOMAS OF SIV-IMMUNOSUPPRESSED MONKEYS, International journal of cancer, 55(4), 1993, pp. 609-615
Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop
B-cell lymphomas in approximately one third of the cases. We have now
studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuc
lear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphoma
s and established cell lines from 3 of these tumors. Immunoblots of ce
ll lysates were probed with polyspecific and monospecific reagents dir
ected against human EB-virus EBNA1-6, and against the membrane protein
LMP1. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma
tissues (8/13) and in the 3 cell lines derived from the tumors. All tu
mors expressed a polypeptide with SO to 55 kDa molecular weight, which
cross-reacted with some antibodies to EBNAI. Absorption experiments w
ith normal monkey tissue showed that this polypeptide was specific for
the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMP1
could not be detected. Immunophenotypical characterization showed tha
t the monkey lymphomas were similar to human HIV-associated B-cell lym
phomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cy
nomolgus monkeys can be a model for EBV-associated lymphomagenesis in
immunodeficiency states. (C) 1993 Wiley-Liss, Inc.