EXPRESSION OF EPSTEIN-BARR-VIRUS-RELATED NUCLEAR ANTIGENS AND B-CELL MARKERS IN LYMPHOMAS OF SIV-IMMUNOSUPPRESSED MONKEYS

Citation
Sl. Li et al., EXPRESSION OF EPSTEIN-BARR-VIRUS-RELATED NUCLEAR ANTIGENS AND B-CELL MARKERS IN LYMPHOMAS OF SIV-IMMUNOSUPPRESSED MONKEYS, International journal of cancer, 55(4), 1993, pp. 609-615
Citations number
37
Categorie Soggetti
Oncology
ISSN journal
00207136
Volume
55
Issue
4
Year of publication
1993
Pages
609 - 615
Database
ISI
SICI code
0020-7136(1993)55:4<609:EOENAA>2.0.ZU;2-S
Abstract
Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuc lear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphoma s and established cell lines from 3 of these tumors. Immunoblots of ce ll lysates were probed with polyspecific and monospecific reagents dir ected against human EB-virus EBNA1-6, and against the membrane protein LMP1. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tu mors expressed a polypeptide with SO to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments w ith normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMP1 could not be detected. Immunophenotypical characterization showed tha t the monkey lymphomas were similar to human HIV-associated B-cell lym phomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cy nomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states. (C) 1993 Wiley-Liss, Inc.