Pj. Loida et Sg. Sligar, MOLECULAR RECOGNITION IN CYTOCHROME-P-450 - MECHANISM FOR THE CONTROLOF UNCOUPLING REACTIONS, Biochemistry, 32(43), 1993, pp. 11530-11538
The pathway for utilization of pyridine nucleotide derived reducing eq
uivalents in the cytochrome P-450 monooxygenase systems has three majo
r branch points. The first is a partitioning between autoxidation of a
ferrous, oxygenated heme adduct and input of the second reducing equi
valent required for monooxygenase stoichiometry. The second is between
dioxygen bond scission and release of two-electron-reduced O2 as hydr
ogen peroxide. The third is between substrate hydrogen abstraction ini
tiated by a putative higher valent iron-oxo species and reduction of t
his intermediate by two additional electrons to produce water in an ov
erall oxidase stoichiometry. For all substrates investigated, the dire
ct release of superoxide at the first branch point never competes with
second electron input. In order to elucidate the aspects of molecular
recognition of a substrate-P-450 complex which affect these individua
l branch points in the catalytic cycle, we have measured the NADH-deri
ved reducing equivalents recovered in hydroxylated substrate, hydrogen
peroxide, and water for a series of active-site mutants designed to a
lter the coupling of ethylbenzene hydroxylation. We find that the reac
tion specificity at the second and third branch points is affected by
site-directed mutations that alter the topology of the binding pocket.
The increased commitment to catalysis observed for all mutants sugges
ts that active-site hydration is important in the uncoupling to form h
ydrogen peroxide at the second branch point. The liberation of hydroge
n peroxide does not correlate with the location of the mutation in the
pocket, as expected if the two-electron-reduced dioxygen-bound interm
ediate is not directly participating in the substrate activation step.
However, a strong correlation is observed between water production at
the third branch point and the location and size of the amino acid si
de chain in the substrate binding site. Larger hydrophobic side chains
introduced in the upper regions of the binding pocket increase the ra
tio of hydroxylated product to water production by 2-4-fold relative t
o wild-type, while similar substitutions in residues near the heme pla
ne result in diminished product. Overall, the partitioning between hyd
roxylation and oxidase activities varies by over 65% due to the locati
on of nonpolar substituents engineered into the active site. Substrate
access to the heme is the key factor for tight coupling at the level
of the putative iron-oxo species. These results are further evidence t
hat a discrete intermediate containing a single oxygen atom (e.g., a f
erryl-oxo complex, [FeO]3+) is the precursor to both substrate hydroxy
lation and the input of two additional electron equivalents to form wa
ter.