Mare granulosa cells and cyclic corpus luteum microsomes are reported
to aromatize 19-norandrogens more efficiently than androgens. However,
16-alpha-hydroxytestosterone and epitestosterone were not aromatized
by the equine corpus luteum microsomal estrogen synthetase. These resu
lts indicate that the equine aromatase system would be different from
the human placental microsomal estrogen synthetase, which aromatizes 1
6-alpha-hydroxyandrogens and epitestosterone but not 19-norandrogens.
Furthermore, our data show that the rates of aromatization of androgen
s and 19-norandrogens were not additive and that 19-norandrogens compe
titively inhibited the aromatization of androgens, suggesting that a s
ingle enzymic system would be involved in the aromatization of androst
enedione, 19-norandrostenedione, testosterone, and 19-nortestosterone.
Our findings, which are identical to those previously reported for st
allion testis and mare placental estrogen synthetases, provide evidenc
e for a strong species specificity of the equine aromatase system.