Cbt. Vandebraak et al., CELLULAR AND HUMORAL CHARACTERISTICS OF PENAEUS-MONODON (FABRICIUS, 1798) HEMOLYMPH, Comparative haematology international, 6(4), 1996, pp. 194-203
To study the usefulness of haemolymph for shrimp health assessment cha
racteristics of both the cellular and humoral composition of growing j
uvenile Penaeus monodon (n = 16) were determined. Haemolymph was obtai
ned by a puncture of the ventral part of the haemocoel. Using routine
staining methods five different cell types could be distinguished by l
ight microscopy. Electron microscopy studies revealed granulocytes, se
migranulocytes and hyalinocytes. Mean total haemocyte count was 50.9 x
10(6) cells/ml whereas mean haemolymph plasma protein content was 79.
9 g/l consisting of one major and one or two minor fractions. It was c
oncluded that haemolymph characterisation can be a useful tool for hea
lth estimation of P. monodon, but standardisation of the techniques is
needed.