Wd. Currie et al., CYTOSOLIC-FREE CA2-RELEASING-HORMONE( IN HUMAN SYNCYTIOTROPHOBLAST CELLS INCREASED BY GONADOTROPIN), Endocrinology, 133(5), 1993, pp. 2220-2226
Effects of GnRH on free cytosolic Ca2+ concentrations ([Ca2+]i) were e
xamined in individual first trimester human cytotrophoblast and syncyt
iotrophoblast cells by fura-2 microspectrofluorimetry. GnRH (10(-6) m)
did not affect [Ca2+]i in cytotrophoblasts on days 2-9 of culture, wi
th 50 cells tested each day. GnRH (10(-6) m) did not affect [Ca2+]i on
days 2-3, but increased [Ca2+]i in 15% of culture-derived syncytiotro
phoblasts on day 4 (8 of 52 cells) and in 48% on days 5-9 of culture (
158 of 332 cells). Culture-derived syncytiotrophoblasts originated fro
m four first trimester placentae. GnRH increased [Ca2+]i in a prelimin
ary trial using syncytiotrophoblasts derived directly from a single fi
rst trimester placenta and cultured for 3 days (13 of 33 cells). Cultu
re-derived first trimester syncytiotrophoblast cells that responded to
10(6) M GnRH (28 of 63 cells) on day 6 also responded to GnRH at 10(-
7) m (26 of 28 of the above cells), 10(-8) m (24 of 28 cells), 10(-9)
m (22 of 28 cells), and 10(-10) m (3 of 28 cells). No cells responded
to GnRH below a concentration of 10(-10) M. Desensitization of syncyti
otrophoblasts by continuous GnRH perifusion (10(-6) m) and blockade of
GnRH by competitive antagonism with Nal-Glu-GnRH (10(-6) m) suggested
that effects of GnRH were receptor specific. The results provide dire
ct evidence supporting the contention that the intracellular signaling
resultant from GnRH receptor-ligand interactions in syncytiotrophobla
sts may be at least partially mediated by transient increases in [Ca2]i.