IMMUNE ALTERATIONS IN MORPHINE-TREATED RHESUS-MONKEYS

The effect of morphine treatment on immunocompetence was investigated in rhesus monkeys (Mucaca mulatta). Monkeys that received morphine dai ly (3.2 mg/kg) had suppressed peripheral blood mononuclear cell (PBMC) natural killer (NK) activity as well as a decrease in the percentage of CD8+CD16+ cells compared with saline-treated (controls) or infreque ntly treated (i.e., opioids once or twice weekly) monkeys. However, an increase (20 +/- 5%) in the percentage of CD8+ lymphocytes was found in both the daily and infrequent opioid-treated monkeys compared with controls. Conversely, the percentage of total CD4+ lymphocytes and CD4 +CD45RA+ was reduced (12 +/- 2% and 28 +/- 10% respectively) in both d aily and infrequent opioid-treated animals compared with saline-treate d controls. In a reciprocal fashion, there was an increase in the CD4CD29+ population in daily morphine-treated monkeys (54% of the total C D4+ cells) compared with untreated animals (37% of the total CD4+ cell s). In addition, cultured PBMC obtained from monkeys treated daily wit h morphine produced significantly (P < .01) more polyclonal immunoglob ulin (1g) G (366 ng/ml) and polyclonal IgM (233 ng/ml) compared with P BMC production of polyclonal IgG (96 ng/ml) and polyclonal IgM (67 ng/ ml) from saline-treated controls. However, no differences were found i n the percentage of CD19+ lymphocytes among any of the groups. In summ ary, daily treatment with a relatively low dose of morphine (3.2 mg/kg ) affects immunocompetence which could have important implications in the regulation of viral pathogens in i.v. drug abusers (e.g., AIDS).