THE MECHANISM OF INTERACTION OF CERULOPLASMIN WITH SUPEROXIDE RADICALS

1. The mechanism of interaction of CP with O2- radicals in chemical an d enzymatic systems of superoxide radical generation as well as in the pulse radiolysis technique was studied. 2. It is found that CP does n ot exert any kinetic influence on the decomposition of superoxide radi cal and, unlike SOD, cannot catalyze the reaction of disproportionatio n of these radicals in systems with chemical and enzymatic generation of O2-. 3. The data obtained confirm the suggestion that CP interacts with precursors of O2- radicals. 4. The irradiation of CP does not cha nge its inhibiting activity in the reaction of the formation of supero xide radicals in systems with enzymatic O2- generation, but decreases its oxidase activity. 5. The results obtained demonstrated that the in crease in the radiation dose resulted in the decrease of the inhibitin g activity of SOD, whereas the activity of CP did not change.