H. Grote et al., LOCALIZATION OF THE GLUCOCORTICOID RECEPTOR IN RAT-LIVER CELLS - EVIDENCE FOR PLASMA-MEMBRANE BOUND RECEPTOR, International Journal of Biochemistry, 25(11), 1993, pp. 1593-1599
1. The cytoplasmic glucocorticoid receptor of rat liver cells is in pa
rt recovered in the plasma membrane fraction. 2. After in vivo adminis
tration of [H-3]dexamethasone, 0.35% of the radioactivity recovered is
bound on plasma membranes. 3. Dexamethasone also binds in vitro speci
fically to plasma membranes. Expressed as fmol/mg protein, binding of
dexamethasone to plasma membranes is comparable to binding to the solu
ble cytoplasmic fraction (cytosol). 4. Using polyclonal antibody to th
e glucocorticoid receptor and the indirect immunofluorescence technic,
an intense decoration of the plasma membranes is observed, denoting a
high concentration of glucocorticoid receptor on plasma membranes. 5.
The localization of the receptor on plasma membranes could be of pote
ntial importance for its interaction with agents (mitogens, growth fac
tors) initially acting on the cell membrane, regulating subsequent cel
l proliferation and growth at the level of the cell nucleus.