S. Premaratne et al., IDENTIFICATION OF DNA-ADDUCTS AT SPECIFIC LOCATIONS BY SEQUENCING TECHNIQUES, International Journal of Biochemistry, 25(11), 1993, pp. 1669-1672
1. A simple procedure for the accurate identification of the positions
of adduct formation when viral M13mp18 single stranded DNA is treated
with chloroacetaldehyde (CAA) is presented. 2. The normal dideoxy seq
uencing reaction protocol was employed except no dideoxy nucleotides w
ere used to cause chain termination. 3. Instead, the CAA adducted base
s of the template DNA act as points of polymerase fall off causing the
appearance of bands of DNA sequencing gel autoradiographs marking loc
ations where adduct formation may have occurred.