IN-VITRO INHIBITORY ACTIVITY OF SOLUBLE ICAM-1 FOR THE NUMBERED SEROTYPES OF HUMAN RHINOVIRUS

We studied the antirhinovirus activity of soluble intercellular adhesi on molecule-1 (sICAM-1) against all 100 numbered human rhinovirus (HRV ) serotypes in human embryonic lung fibroblast (HEL) cells. sICAM-1 in hibited replication of 88 of 90 HRVs belonging to the major receptor g roup with 50% effective concentrations (EC50) ranging from 0.1 to 41.1 mug ml-1 (approximately 0.002 to 0.8 mum). No inhibition at 100 mug m l-1 (approximately 2 mum) was observed for HRV belonging to the minor receptor group, type 87, and two serotypes of the major receptor group (types 23 and 25). Yield reduction assays done with five HRV serotype s in human adenoid explants found that sICAM-1 had concentration-depen dent inhibitory effects that correlated closely with the activity obse rved in HEL cells (r2 = 0.99, P < 0.001).