INHERITANCE AND LINKAGE RELATIONSHIPS OF ISOZYME VARIANTS OF PINUS-LEUCODERMIS ANT

Inheritance and linkage relationships of 13 enzyme systems were invest igated by horizontal starch gel electrophoresis in female gametophytes of Pinus leucodermis ANT.. Isozymes observed were under the control o f at least 23 loci. In 2 of these loci, GDH and NDH, no variation was detected. The segregation of allozymes in megagametophytes of heterozy gous trees revealed simple Mendelian inheritance for most of the enzym e systems analyzed. Distorted segregation ratios were observed at ACO, GOT and LAP loci. Linkages were studied by analyzing 61 2-locus combi nations. Significant linkage, as attested by the 95% confidence interv als, was detected for GOT-A:PGI-B, G6PD-A:LAP-B, LAP-B:PGM-A and 6PGD- B:PGI-B. Estimates of recombination frequencies varied trees. The tigh test association was observed between 6PGD-B and PGI-B loci, with a re combination frequency of 8.1%.