SUPPRESSION OF PHASEOLIN AND LECTIN IN SEEDS OF COMMON BEAN, PHASEOLUS-VULGARIS L - INCREASED ACCUMULATION OF 54 KDA POLYPEPTIDES IS NOT ASSOCIATED WITH HIGHER SEED METHIONINE CONCENTRATIONS
Md. Burow et al., SUPPRESSION OF PHASEOLIN AND LECTIN IN SEEDS OF COMMON BEAN, PHASEOLUS-VULGARIS L - INCREASED ACCUMULATION OF 54 KDA POLYPEPTIDES IS NOT ASSOCIATED WITH HIGHER SEED METHIONINE CONCENTRATIONS, MGG. Molecular & general genetics, 241(3-4), 1993, pp. 431-439
Suppression of phaseolin and lectin accumulation in common bean result
ed in higher concentrations of bean seed polypeptides with apparent mo
lecular weights of 54 kDa and from 70 to 84 kDa on SDS-polyacrylamide
gel electrophoresis. Polypeptides of 54 and 56 kDa segregated as produ
cts of different alleles. Genes for the 54/56 kDa bands and phaseolin
were estimated to be 26.2 +/- 3.7 map units apart. The 54 kDa band phe
notype manifested by SDS-PAGE consisted of from one to three polypepti
des of 54 kDa MW on 2D gels, and the 56 kDa phenotype consisted of one
polypeptide of 56 kDa plus two minor polypeptides of 54-54.5 kDa mole
cular weight. The pK(I) of these polypeptides was approximately 5.25.
The methionine content of the 54 kDa polypeptides of the cultivar Grea
t Northern Star was 1.6 +/- 0.1 g/100 g protein, which was not statist
ically different from the value (1.5 +/- 0.1%) obtained for phaseolin
isolated by the same procedure. F2 seeds deficient for phaseolin and l
ectin contained as much total N per g as wild-type seeds and were not
shrunken, but contained 50% more free amino acids. F2 seeds from two o
f the three populations contained from 8 to 13% less methionine per mg
total N.