THE EFFECTS OF PEG ON 2ND ANTIBODY IMMUNOPRECIPITATION AND ITS USE INIMMUNOASSAY

The effects of PEG on the second antibody immunoprecipitation have bee n studied in several radioimmunological systems. The following paramet ers have been studied in the assays: the average molecular weight and concentration of PEG, the species of the animal producing the first an d second antibody, the nature of the antigen, the concentration of car rier immunoglobulins, and the rate of the immunoprecipitation. Thus, o ptimal conditions for the use of PEG with a second antibody have been defined in order to be used in any liquid