AMPHOLYTE-FREE ISOELECTRIC-FOCUSING OF PROTEINS IN CONE-SHAPED CAPILLARIES

A simple method of generating pH gradients for use in capillary isoele ctric focusing was described and evaluated experimentally. In the prop osed scheme, the cone shaped capillary filled with a conventional buff er is used to produce an effect of nonuniform heating which then gener ate temperature and corresponding pH gradients along the capillary axi s. Concentration and separation of proteins by applying a simple isoel ectric focusing system based on this principle is demonstrated. An ine xpensive absorption imaging detection system is used to continuously m onitor progress of the separation and concentration processes.