MULTIDATA TREATMENT APPLIED TO THE SIMULTANEOUS RESOLUTION OF CATECHOL RESORCINOL MIXTURES BY KINETIC ENZYMATIC PROCESSES

Citation
E. Gomez et al., MULTIDATA TREATMENT APPLIED TO THE SIMULTANEOUS RESOLUTION OF CATECHOL RESORCINOL MIXTURES BY KINETIC ENZYMATIC PROCESSES, Talanta, 40(11), 1993, pp. 1601-1607
Citations number
17
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
40
Issue
11
Year of publication
1993
Pages
1601 - 1607
Database
ISI
SICI code
0039-9140(1993)40:11<1601:MTATTS>2.0.ZU;2-9
Abstract
This paper reports a multicomponent least-squares regression method ba sed on the use of multiple standards for the simultaneous resolution o f substrates involved in kinetic enzymatic processes. The proposed met hod was applied to the determination of catechol-resorcinol mixtures b y oxidation with hydrogen peroxide in the presence of the enzyme perox idase (EC 1.11.1.7) in a stopped-flow reversed flow injection system. The mathematical algorithm used is superior to other alternatives as i t is not affected by side reactions between the oxidation products. Th e proposed method allows the simultaneous resolution of 50-150muM cate chol and 30-180muM resorcinol over the wavelength range 340-500 nm. Th e concentration ranges can be modified by varying the injected amount of enzyme. The mathematical treatment is applied to the spectra of sev eral standards and those of the samples, which are recorded 6 sec afte r the flow is stopped. This redounds to a high sampling frequency (up to 60/hr).