HUMAN ALPHA-SATELLITE DNAS ARE NOT SUSCEPTIBLE TO UNDERCONDENSATION AFTER 5-AZACYTIDINE TREATMENT

Authors
FERNANDEZ JL GOYANES V LOPEZFERNANDEZ C GOSALVEZ J
Citation
Jl. Fernandez et al., HUMAN ALPHA-SATELLITE DNAS ARE NOT SUSCEPTIBLE TO UNDERCONDENSATION AFTER 5-AZACYTIDINE TREATMENT, Cytogenetics and cell genetics, 65(1-2), 1994, pp. 92-94
Citations number
13
Categorie Soggetti
Cytology & Histology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
65
Issue
1-2
Year of publication
1994
Pages
92 - 94
Database
ISI
SICI code
0301-0171(1994)65:1-2<92:HADANS>2.0.ZU;2-7
Abstract
Localization of alphoid human satellite DNAs using fluorescence in sit u hybridization (FISH) of metaphase chromosomes following treatment wi th 5-azacytidine to produce undercondensation showed that human alpha- satellite DNA is not sensitive to the condensation-inhibition effect o f 5-azacytidine. The difference in heterochromatic DNA subsets was par ticularly evident in the constitutive heterochromatin of chromosomes 1 and 9. Comparison of the results obtained after FISH with those obtai ned from electron microscopy and G-banding enabled accurate localizati on of this DNA domain.