SEQUENCE-INDEPENDENT AMPLIFICATION AND LABELING OF YEAST ARTIFICIAL CHROMOSOMES FOR FLUORESCENCE IN-SITU HYBRIDIZATION

Authors
BOHLANDER SK ESPINOSA R FERNALD AA ROWLEY JD LEBEAU MM DIAZ MO
Citation
Sk. Bohlander et al., SEQUENCE-INDEPENDENT AMPLIFICATION AND LABELING OF YEAST ARTIFICIAL CHROMOSOMES FOR FLUORESCENCE IN-SITU HYBRIDIZATION, Cytogenetics and cell genetics, 65(1-2), 1994, pp. 108-110
Citations number
14
Categorie Soggetti
Cytology & Histology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
65
Issue
1-2
Year of publication
1994
Pages
108 - 110
Database
ISI
SICI code
0301-0171(1994)65:1-2<108:SAALOY>2.0.ZU;2-P
Abstract
We have developed a method that allows reliable construction of high q uality FISH probes from yeast artificial chromosomes (YACs) based on t he separation of YACs by pulsed-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can b e used to localize YACs on metaphase chromosomes and also with high ef ficiency, in interphase nuclei.