HIGH-VOLTAGE ELECTROPHORESIS OF AMINO-ACIDS IN URINE CONTAINING AMPICILLIN

Authors
THUY LP NYHAN WL
Citation
Lp. Thuy et Wl. Nyhan, HIGH-VOLTAGE ELECTROPHORESIS OF AMINO-ACIDS IN URINE CONTAINING AMPICILLIN, Clinical biochemistry, 26(5), 1993, pp. 389-390
Citations number
3
Categorie Soggetti
Biology,"Chemistry Medicinal
Journal title
Clinical biochemistry → ACNP
ISSN journal
00099120
Volume
26
Issue
5
Year of publication
1993
Pages
389 - 390
Database
ISI
SICI code
0009-9120(1993)26:5<389:HEOAIU>2.0.ZU;2-H
Abstract
Analysis of amino acids is complicated by treatment with ampicillin. H igh voltage electrophoresis, which is convenient for the qualitative a ssessment of metabolic diseases, yields smears of ampicillin that mask the bands of citrulline, homocitrulline, phenylalanine, cystine, and homocystine. The addition of penicillinase prior to high voltage elect rophoresis eliminates ampicillin and other penicillins and reveals the se key amino acids.