DETECTION OF GENOMIC ALTERATIONS IN HUMAN CERVICAL-CANCER BY 2-DIMENSIONAL GEL-ELECTROPHORESIS

Two-dimensional gel electrophoresis was used to comprehensively scan t he whole genome of 6 cervical intraepithelial neoplasia (CIN) lesions, 7 cervical squamous cell carcinomas, 1 cervical adenosquamous cell ca rcinoma, and 2 cervical adenocarcinomas for multiple genetic alteratio ns, such as DNA amplification, chromosome deletion, loss of heterozygo sity, and chromosome translocation, as compared with the paired normal tissues. DNA spot analysis of the genomic 2-dimensional gels was perf ormed by a computer color overlay system and by spot recognition softw are allowing for objective spot comparison and quantitation. Nine spot s were found to be amplified in the cervical carcinomas while two ampl ified spots were detected in the CIN III lesions. Fourteen DNA spots w ere either reduced in their intensity or absent in cervical carcinomas as compared to their normal paired tissues. Reduction of intensity in 6 spots was observed in the 5 CIN III lesions. These genetic alterati ons may represent changes in cancer genes that are associated with hum an cervical carcinogenesis. Further characterization of these alterati ons may be significant to the understanding of cervical tumorigenesis and to the development of biomarkers for clinical trials in cancer che moprevention. (C) 1997 Wiiey-Liss, Inc.