RESTRICTION FRAGMENT PATTERNS OF CHLOROPLAST AND MITOCHONDRIAL-DNA OFDASYPYRUM-VILLOSUM (L) CANDARGY AND WHEATS

To elucidate the phylogenetic relationships and cytoplasmic types, res triction endonuclease fragment patterns of chloroplast (cp) and mitoch ondrial (mt) DNAs isolated from two different accessions of Dasypyrum villosum (L.) candargy were compared with those of tetraploid wheat (T riticum durum Desf., PI265007), hexaploid wheat (Triticum aestivum L., cv Chinese Spring), Aegilops longissimum (S. and M., in Muschli) Bowd en and Hordeum vulgare L. T aestivum and T durum had identical restric tion patterns for their cp and mtDNAs in digestions with four differen t enzymes. Likewise, no differences were found between the restriction fragment patterns of two accessions of D. villosum. But, there were d istinct differences in chloroplast and mitochondrial DNA restriction f ragment patterns between D. villosum and tetraploid and hexaploid whea ts. A. longissimum (G609) showed a similar pattern to those wheats for PstI digestion of cpDNA. Organellar DNA from Hordeum vulgare (cv Hima laya) showed a distinctly different restriction pattern from those of wheat and D. villosum. These results suggest that D. villosum is unlik ely to be the donor of cytoplasm to wheats, and its cytoplasmic organe lles were also different from those of A. longissimum.