Sm. Kaeppler et al., USE OF NEAR-ISOGENIC LINES DERIVED BY BACKCROSSING OR SELFING TO MAP QUALITATIVE TRAITS, Theoretical and Applied Genetics, 87(1-2), 1993, pp. 233-237
Near-isogenic lines (NILs) are a valuable resource for detecting linka
ges between qualitative trait loci and molecular markers. Molecular ma
rker studies are expensive and methods that require genotyping fewer i
ndividuals, such as the NIL-analysis method, are desirable. We present
a theory for using sets of NILs to detect linkages between molecular
markers and introgressed loci. The probability that a marker a specifi
c distance from the introgressed gene will have a donor parent allele
in a near-isogenic line is a function of the distance between the mark
er and the gene, and the number of backcrosses and/or selfs used in de
riving the NIL. The binomial probability formula is used to calculate
the probability of having a donor parent allele at a given marker when
sets of NILs are used. The formulae given allow calculation of the pr
obability that a marker is linked to the introgressed gene, as well as
the probability that a gene will be successfully detected when using
given numbers of NILs, backcrosses, and molecular markers.