Roc. Oreffo et al., CHARACTERIZATION OF A CELL-LINE DERIVED FROM A HUMAN GIANT-CELL TUMORTHAT STIMULATES OSTEOCLASTIC BONE-RESORPTION, Clinical orthopaedics and related research, (296), 1993, pp. 229-241
Giant cell tumors of bone are common but unusual tumors that are compr
ised of multiple cell types. Most attention has been focused on the gi
ant cells, which resemble osteoclasts morphologically and functionally
. This study examines the properties of a cell line derived from monon
uclear cells from one of these tumors, since it appears likely that th
ese cells may be able to influence the activities of cells with the os
teoclast phenotype. This cell line, C433, hast be following characteri
stics: (1) it represents undifferentiated cells, not recognized by any
known antigenic markers for leukocytes; (2) it contains tartrate-resi
stant acid phosphatase; (3) it responds to the osteotropic factors 1,2
5 dihydroxyvitamin D3, insulin-like growth factor I and II, but not to
parathyroid hormone; (4) it forms sarcomas in nude mice; and (5) it p
roduces an activity that stimulates isolated avian and rat osteoclasts
to resorb bone. This cell line may be useful in examining interaction
s between osteoclasts and accessory cells involved in bone resorption.