E. Reiss et T. Bryngelsson, PATHOGENESIS-RELATED PROTEINS IN BARLEY LEAVES, INDUCED BY INFECTION WITH DRECHSLERA-TERES (SACC) SHOEM AND BY TREATMENT WITH OTHER BIOTIC AGENTS, Physiological and molecular plant pathology, 49(5), 1996, pp. 331-341
The perthotrophic fungus Drechslera teres, the causal agent of net blo
tch disease in barley, induces the accumulation of pathogenesis-relate
d (PR) proteins in barley leaves as shown by isoelectric focusing. The
same protein pattern was also found in leaves treated with a toxin ex
tract from the culture filtrate of D. teres as well as after infection
with Erysiphe graminis f.sp. hordei or Puccinia hordei. Some of the p
roteins induced by infection with D. teres were characterized as perox
idases, beta-1,3-glucanases and chitinases by isoenzyme analysis. Immu
nodetection following western blots demonstrated that the induced prot
eins are the same as those that accumulate after inoculation of barley
with E. graminis: basic PR-1a and b proteins, thaumatin-like (TL) pro
teins, beta-1,3-glucanases and chitinases. The accumulation of PR-1 ty
pe proteins, chitinases and TL-proteins was analysed quantitatively by
ELISA. (C) 1996 Academic Press Limited.