CHARACTERIZATION OF THE C-ALPHA-DEHYDROGENASE GENE INVOLVED IN THE CLEAVAGE OF BETA-ARYL ETHER BY PSEUDOMONAS-PAUCIMOBILIS

Authors
MASAI E KUBOTA S KATAYAMA Y KAWAI S YAMASAKI M MOROHOSHI N
Citation
E. Masai et al., CHARACTERIZATION OF THE C-ALPHA-DEHYDROGENASE GENE INVOLVED IN THE CLEAVAGE OF BETA-ARYL ETHER BY PSEUDOMONAS-PAUCIMOBILIS, Bioscience, biotechnology, and biochemistry, 57(10), 1993, pp. 1655-1659
Citations number
20
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
57
Issue
10
Year of publication
1993
Pages
1655 - 1659
Database
ISI
SICI code
0916-8451(1993)57:10<1655:COTCGI>2.0.ZU;2-Q
Abstract
Calpha-dehydrogenase catalyzes the oxidation of arylglycerol-beta-aryl ether at the Ca-position, and therefore this process produces the spe cific substrate for beta-etherase, which cleaves beta-ary ethers (Calp ha carbonyl type). Here we isolated the Calpha-dehydrogenase gene (lig D) and sequenced its nucleotides. This gene contains an open reading f rame of 915 bp and the deduced amino acid sequence had a homology with the ribitol dehydrogenase family. ligD is about 1 kbp upstream of the beta-etherase gene (ligE).