ENZYMATIC-SYNTHESIS OF (-ALPHA-GLUCOSIDE AND ITS EFFECT ON TYROSINASEACTIVITY()CATECHIN)

Authors
FUNAYAMA M NISHINO T HIROTA A MURAO S TAKENISHI S NAKANO H
Citation
M. Funayama et al., ENZYMATIC-SYNTHESIS OF (-ALPHA-GLUCOSIDE AND ITS EFFECT ON TYROSINASEACTIVITY()CATECHIN), Bioscience, biotechnology, and biochemistry, 57(10), 1993, pp. 1666-1669
Citations number
9
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
57
Issue
10
Year of publication
1993
Pages
1666 - 1669
Database
ISI
SICI code
0916-8451(1993)57:10<1666:EO(AIE>2.0.ZU;2-T
Abstract
The glycosidation of (+)catechin, which has five hydroxyl groups with cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) and soluble star ch has been studied. One of the transfer products was purified and its structure was determined to be (+)catechin 3'-O-alpha-D-glucopyranosi de. This glucoside noncompetitively inhibited the activity of tyrosina se from mushroom, IC50 being 5.8 mM, but didn't inhibit that from mous e melanoma. In contrast, arbutin (hydroquinone-O-beta-D-glucopyranosid e) inhibited both tyrosinases.