FLAGELLAR FORMATION DEPENDS ON MEMBRANE ACIDIC PHOSPHOLIPIDS IN ESCHERICHIA-COLI

Authors
NISHINO T KITAMURA E MATSUZAKI H NISHIJIMA S MATSUMOTO K SHIBUYA I
Citation
T. Nishino et al., FLAGELLAR FORMATION DEPENDS ON MEMBRANE ACIDIC PHOSPHOLIPIDS IN ESCHERICHIA-COLI, Bioscience, biotechnology, and biochemistry, 57(10), 1993, pp. 1805-1808
Citations number
29
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
57
Issue
10
Year of publication
1993
Pages
1805 - 1808
Database
ISI
SICI code
0916-8451(1993)57:10<1805:FFDOMA>2.0.ZU;2-O
Abstract
Escherichia coli pgsA3 mutants deficient in acidic phospholipids were found to be defective in flagellar formation. This was first noticed b y the unusual protein patterns of outer membrane fractions and was con firmed by measuring whole-cell total flagellin. pgsA3 mutants were com pletely nonmotile. The defects were suppressed by a plasmid carrying t he pgsA coding region. Flagellin levels and motility of null cls mutan ts that lacked cardiolipin synthase were almost normal. We conclude th at the flagellum synthesis in E. coli is dependent on the membrane lev els of acidic phospholipids.