A varied population of Cucumber Mosaic Virus (CMV) isolates is reporte
d from peppers (Capsicum annuum) in Australia. Isolates representing b
oth CMV subgroups (serogroups) I and II have been obtained from the sa
me field at the same sampling time. The CMV isolates were typed into s
ubgroup I (eight isolates) and subgroup II (two isolates) using both a
nucleic acid hybridization assay and an immunological assay with mono
clonal antibodies. The immunological assay described allows the typing
of strains in crude sap extracts, obviating the need for purified vir
ions. The spatial and temporal coincidence of both CMV subgroups prese
nts a situation in which pseudorecombinants with reassorted genomic co
mponents might arise.