BIOSENSOR FOR DETERMINATION OF STARCH

Biosensor for determination of starch was prepared by fixing a carrier with immobilized enzyme system on the tip of a Clark-type oxygen sens or. Enzymes amyloglucosidase, mutarotase, glucose oxidase and catalase were co-immobilized either on the partially hydrolyzed nylon net or o n the activated collagen membrane. In both cases the covalent bond was made in the presence of glutaraldehyde and cyclohexyl isocyanide. The prepared biosensors were compared from the view-point of a specific a ctivity of the immobilized amyloglucosidase, of a linear response area of the biosensor to the substrate concentration, of the pH and temper ature of the reaction mixture and of the biosensor stability. Biosenso rs prepared were used for the determination of starch in the samples o f cultivaiting media for the monensin production.