EFFECTS OF POLYAMINES ON A CONTINUOUS CELL-FREE PROTEIN-SYNTHESIS SYSTEM OF AN EXTREME THERMOPHILE, THERMUS-THERMOPHILUS

A continuous cell-free protein synthesis system of an extremely thermo philic eubacterium, Thermus thermophilus HB27, was constructed. This s ystem produced MS2 phage RNA translation products at a rate of more th an 5 mu g per hour per 1.9 mg of ribosomes at 65 degrees C and the pro duction continued linearly for at least 340 min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when O.1 mM tetrakis(3-aminopropyl)ammonium and 1.0 mM sp ermine were added simultaneously.