REVERSION OF UV-LIGHT MORPHOLOGICAL AND COLOR MUTANTS OF TRICHODERMA-VIRIDE

Authors
Citation
A. Fargasova, REVERSION OF UV-LIGHT MORPHOLOGICAL AND COLOR MUTANTS OF TRICHODERMA-VIRIDE, Acta microbiologica Hungarica, 39(3-4), 1992, pp. 249-256
Citations number
7
Categorie Soggetti
Microbiology
ISSN journal
02314622
Volume
39
Issue
3-4
Year of publication
1992
Pages
249 - 256
Database
ISI
SICI code
0231-4622(1992)39:3-4<249:ROUMAC>2.0.ZU;2-V
Abstract
By using UV light 13 years ago from Trichoderma viride 8 - 7 Pers. ex S. F. Gray 82 colour and morphological mutants were prepared. The reve rsion of these mutants was high and in 1985 only 26 stable mutants (i. e. 32%) could be used for genetic studies. In 1991, from these 26 muta nts we had only 6 stable types (i.e. 7.3% from the original state). Th ese mutants were permanently transferred on fresh CzDA medium every 3- 4 months and checked for morphology, growth, conidiation, heterokaryon s and diploids production. The group of mentioned mutants was enlarged with mutants which were after 3-4 transfers on fresh medium, preserve d dried to the present time. The largest differences were observed in microscopic appearance of colonies. Differences between growth rates, intensity of conidiation were comparable with those made 13 years ago.