CHLOROQUINE INHIBITS THE INSULIN BINDING AND THE IMPRINTING OF NUCLEAR-ENVELOPE IN TETRAHYMENA

Chloroquine possessing lysosomotrop effects inhibits the development o f insulin imprinting both on the plasma membrane and the nuclear envel ope. Simultaneously chloroquine can inhibit the insulin binding in the nucleus itself but not in the plasma membrane. There is a dose depend ent increase of binding of labelled insulin to the nuclear envelope. T he control related binding of nuclei pretreated with insulin or chloro quine is similar and have a direct ratio, independently of the applied concentrations.