EVALUATION OF INDIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF COXSACKIEVIRUSES IN CLINICAL-SAMPLES AND ITS COMPARISON WITH DOT-IMMUNOBINDING ASSAY

Indirect enzyme linked immunosorbent assay (ELISA) was applied for the direct detection of coxsackieviruses in clinical samples viz. rectal swabs (RS) and throat swabs (TS) collected from patients admitted to v arious nursing homes and local hospitals. Results indicate the presenc e of different CVA types in 65 (62.5%) out of 104 RS, and 18 (52.9%) o ut of 34 TS samples. Dot-immunobinding assay was also standardized for the identification of CVA types employing 52 RS samples and the resul ts compared with indirect ELISA. Dot-immunobinding detected more CVA t ypes in a relatively larger number of specimens than indirect ELISA.