PROSTAGLANDIN-E(2) ANTAGONIZES GINGIVAL FIBROBLAST PROLIFERATION STIMULATED BY INTERLEUKIN-1-BETA

Treatment of human gingival fibroblasts with prostaglandin E2 (PGE2) r esulted in significant concentration-dependent inhibition in deoxyribo nucleic acid (DNA) synthesis (8.40-37.89%), while indomethacin (INDO) (PG inhibitor), interleukin-1beta (IL-1beta) or IL-1beta + INDO caused a significant and dose-dependent increase in DNA synthesis. Addition of PGE2 to culture media containing IL-1beta and INDO caused a signifi cant concentration-dependent reduction in IL-1beta- and INDO-induced s timulation of DNA synthesis. The findings suggest that IL-1beta and PG E2, which are also produced by fibroblasts, could play an important ro le in regulation of gingival tissue development and wound healing, and their modulation may have therapeutic potential.