ENZYME IMMUNOASSAYS FOR OVARIAN-STEROID METABOLITES IN THE URINE OF MACACA-FASCICULARIS

Authors
SHIDELER SE SHACKLETON CHL MORAN FM STAUFFER P LOHSTROH PN LASLEY BL
Citation
Se. Shideler et al., ENZYME IMMUNOASSAYS FOR OVARIAN-STEROID METABOLITES IN THE URINE OF MACACA-FASCICULARIS, Journal of medical primatology, 22(5), 1993, pp. 301-312
Citations number
13
Categorie Soggetti
Zoology,"Veterinary Sciences
Journal title
Journal of medical primatology → ACNP
ISSN journal
00472565
Volume
22
Issue
5
Year of publication
1993
Pages
301 - 312
Database
ISI
SICI code
0047-2565(1993)22:5<301:EIFOMI>2.0.ZU;2-8
Abstract
In vivo studies using carbon 14 labeled estradiol (E(2)) and progester one (Po) were performed to characterize the time course and metabolic fate of circulating E(2) and Po. Co-chromatography of human, orangutan , and macaque luteal phase urine samples demonstrated the presence of a steroid conjugate peak in all three species that was identified as b eing androsterone and etiocholanolone glucuronides. An enzyme immunoas say for urinary metabolites of Po was developed subsequently for Macac a spp. using a monoclonal antibody that cross-reacted with both C-19 a nd C-21 metabolites.