BIOACCUMULATION OF DEHYDROABIETIC ACID IN RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) - ROLE OF BIOTRANSFORMATION ENZYMES

The role of Phase I and Phase II metabolism in rainbow trout on the bi oaccumulation of dehydroabietic acid, an abundant and persistent compo und found in pulp and paper mill effluents, was investigated using kno wn inducer/inhibitor compounds for cytochromes P450 (P450s), ethoxyres orufin O-deethylase (EROD) and uridine diphosphoglucuronosyltransferas e (UDPGT). The inducer/inhibitor compounds consisted of piperonyl buto xide (PBO), salicylamide (SAL) and beta-naphthoflavone (beta NF). PBO induced EROD, SAL had no effect on the measured biotransformation enzy me activities and beta NF induced EROD and UDPGT. Treatment of trout w ith these compounds altered the bioaccumulation and distribution of de hydroabietic acid (DHAA) between bile, liver, and muscle in trout expo sed to both the pure compound and an untreated mill effluent indicatin g that biotransformation enzyme activities may directly influence thes e bioconcentration processes in recipient biota. (C) 1997 IAWQ.