DIRECT ASSAY OF TINIDAZOLE IN HUMAN SERUM BY MICELLAR LIQUID-CHROMATOGRAPHY

A liquid chromatographic procedure for the direct determination of tin idazole in human serum is presented, It includes the use of a micellar mobile phase consisting of SDS (5.10(-2) ha): propan-1-ol; (94:6; v/v ) and a mu Bondapak CN column with UV detection at 320 nm, No solvent extraction or deproteinization are necessary, The linearity (0.1-10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99% ), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed-phase HPLC are used, One hundred injections of serum samples do not affect the c olumn life, The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole.